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Team:Newcastle/16 August 2010
From 2010.igem.org
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==Methods== | ==Methods== | ||
| - | # The dehydrated DNA is resuspended in 0. | + | # The dehydrated DNA is resuspended in 0.5 ml of water. |
# We then find out the concentration of resuspended ''yneA'' from [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]]. | # We then find out the concentration of resuspended ''yneA'' from [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]]. | ||
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=Transformation of ''E. coli''= | =Transformation of ''E. coli''= | ||
Revision as of 08:57, 19 August 2010
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Contents |
Rehydrating yneA
Aim
To get yneA hydrated so that we can run restriction digest (see below).
Methods
- The dehydrated DNA is resuspended in 0.5 ml of water.
- We then find out the concentration of resuspended yneA from nanodrop.
Transformation of E. coli
Aim
Transforming yneA into E. coli so that we have plenty of stocks to future tests.
Materials and Protocol
Please refer to transformation of E. coli
Results and Conclusion
Please refer to 17.08.10.
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