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TeamNewcastleNanoDrop Spectrophotometer
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# Log onto computer and select Nanodrop program from the desktop (ND 1000) | # Log onto computer and select Nanodrop program from the desktop (ND 1000) | ||
# Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean. | # Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean. | ||
| - | [[Image:drop.jpg|350px]][[Image:drop2. | + | [[Image:drop.jpg|350px]][[Image:drop2.jpg|350px]] |
# After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (Example: Miniprep samples should be equalized with EB buffer). | # After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (Example: Miniprep samples should be equalized with EB buffer). | ||
# Use DNA-50 for DNA samples. | # Use DNA-50 for DNA samples. | ||
Revision as of 09:44, 12 August 2010
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NanoDrop Spectrophotometer
Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein. The ideal concentration of DNA is 150 ng/ml.
Materials required
- Pipettes
- Nanodrop machine
- Appropriate blanking solution
- Appropriate samples
Procedures
- Log onto computer and select Nanodrop program from the desktop (ND 1000)
- Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.
- After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (Example: Miniprep samples should be equalized with EB buffer).
- Use DNA-50 for DNA samples.
- Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
- If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
- After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.
Go back to our Protocol List
   
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