Team:Calgary/1 August 2010
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- | Today I performed a plasmid switch of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells. | + | Today I performed a plasmid switch of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells and plated. |
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Revision as of 16:56, 5 August 2010
![University of Calgary iGEM Team 2010](http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Header4.png)
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Sunday August 1, 2010
Who wants to work on weekends? We do!!
Chris
Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI.
Dev
Today I performed a plasmid switch of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells and plated.
No notebook page exists for this date. Sorry!