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Team:Calgary/1 August 2010
From 2010.igem.org
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'''Sunday August 1, 2010''' | '''Sunday August 1, 2010''' | ||
| - | Who wants to work on weekends? | + | Who wants to work on weekends? We do!! |
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| + | <u>Chris</u> | ||
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| + | Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI. | ||
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Revision as of 16:01, 3 August 2010
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Sunday August 1, 2010
Who wants to work on weekends? We do!!
Chris
Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI.
No notebook page exists for this date. Sorry!
Retrieved from "http://2010.igem.org/Team:Calgary/1_August_2010"
