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Team:Newcastle/5 July 2010
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===Materials=== | ===Materials=== | ||
| - | * Refrigerated [[Team:Newcastle/1_July_2010#LacI_Biobrick_Construction|transformation plates]] | + | * Refrigerated [[Team:Newcastle/1_July_2010#LacI_Biobrick_Construction|transformation plates]]. |
===Protocol=== | ===Protocol=== | ||
| - | * Set up a liquid culture of colonies transformed with pMutin4 and pSB1AT3 | + | * Set up a liquid culture of colonies transformed with pMutin4 and pSB1AT3. |
===Inference=== | ===Inference=== | ||
| - | *Transformed ''Ecoli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction | + | *Transformed ''Ecoli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction. |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 11:00, 2 August 2010
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Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Materials
- Refrigerated transformation plates.
Protocol
- Set up a liquid culture of colonies transformed with pMutin4 and pSB1AT3.
Inference
- Transformed Ecoli DH5α grown up overnight to create a large stock of plasmid ready for extraction.
   
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