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Team:Newcastle/5 July 2010
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===Materials=== | ===Materials=== | ||
| - | * Refrigerated transformation plates | + | * Refrigerated [[Team:Newcastle/1_July_2010#LacI_Biobrick_Construction|transformation plates]] |
===Protocol=== | ===Protocol=== | ||
Revision as of 10:59, 2 August 2010
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Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Materials
- Refrigerated transformation plates
Protocol
- Set up a liquid culture of colonies transformed with pMutin4 and pSB1AT3
Inference
- Transformed Ecoli DH5α grown up overnight to create a large stock of plasmid ready for extraction
   
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