"
Team:Newcastle/27 July 2010
From 2010.igem.org
(Difference between revisions)
(→Conclusion) |
(→Conclusion) |
||
| Line 15: | Line 15: | ||
====Conclusion==== | ====Conclusion==== | ||
| - | The experiment was successful | + | The experiment was successful; the content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010. |
==='''Gel Electrophoresis'''=== | ==='''Gel Electrophoresis'''=== | ||
Revision as of 19:04, 27 July 2010
| |||||||||||||
| |||||||||||||
Contents |
Genomic DNA extraction experiment
Aims
The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming biobrick and rocF biobrick will then hopefully be obtained from the genomic DNA using...
Protocol
- Please refer to DNA extraction of Bacillus subtilis
Discussion
At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.
Conclusion
The experiment was successful; the content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.
Gel Electrophoresis
Procedure
- Please refer to Gel Electrophoresis
Discussion
- No band was observed on the gel.
Conclusion
The PCR did not work. It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.
   
|
    
|
