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Team:Newcastle/Meetings/26 July 2010
From 2010.igem.org
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==Research feedback== | ==Research feedback== | ||
# Levan glue - Levan requires sucrose to work | # Levan glue - Levan requires sucrose to work | ||
| - | * Test how much sucrose is needed - Phil | + | #* Test how much sucrose is needed - Phil |
# Kill switch - Use of ''mazE'' and ''mazF'' | # Kill switch - Use of ''mazE'' and ''mazF'' | ||
| - | * How much sucrose is needed for it to work? | + | #* How much sucrose is needed for it to work? |
| - | * How fast does the bug use up all the sucrose? | + | #* How fast does the bug use up all the sucrose? |
# Stochastic switch - Use of ''hinC'' as the invertase | # Stochastic switch - Use of ''hinC'' as the invertase | ||
| - | * Matt to work with Phil to work out the probability of the switching | + | #* Matt to work with Phil to work out the probability of the switching |
| - | * Have to work out what is wrong with the iGEM 2009 stochastic switch first | + | #* Have to work out what is wrong with the iGEM 2009 stochastic switch first |
# Gibson protocol | # Gibson protocol | ||
| - | * ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first | + | #* ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first |
==Lab feedback== | ==Lab feedback== | ||
Revision as of 14:44, 27 July 2010
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Contents |
Roll call
Everyone here except Colin Harwood, Colin Davie and Wendy Smith
Research feedback
- Levan glue - Levan requires sucrose to work
- Test how much sucrose is needed - Phil
- Kill switch - Use of mazE and mazF
- How much sucrose is needed for it to work?
- How fast does the bug use up all the sucrose?
- Stochastic switch - Use of hinC as the invertase
- Matt to work with Phil to work out the probability of the switching
- Have to work out what is wrong with the iGEM 2009 stochastic switch first
- Gibson protocol
- rocF to require 6 pair of primers, therefore try doing up 2 fragments first
Lab feedback
- Arginine test - Alan and Harsh
- Test worked fine.
- Could refine the test by using universal liquid pH indicator or to use the pH meter.
- DNA extraction - Harsh and Racheal
- Could only confirm the result on 27.07.2010.
Wiki
- Not able to do the drop down menue with the shadow test, therefore could try using htm5.
- Horizontal strip to change to purple.
Visas
- Deena to travel to London on 12.08.2010.
Action point
- Talk to Lula - Phil and Harsh
- Email to iGEM HQ to get stickers and badges - Harsh
- Hyperlink the protocol page to the lab notebook
- Lab wiki checker - Alan and Harsh
- Send reminder to Jen to add Jane to the mailing list - Jannetta
- Put CBCB and Biomedical school logo onto the wiki - Janetta
- Write to Chris Voigt to get the source code - Steven
- Tutorial for Inkscape - Jannetta
- To check if the extra USA visa immigration form is valid for 2 years - Jen
- Remind Neil about the MrGene logo - Steven
Next meeting
- Phil is chair, Alan is computer, Racheal is minutes
- Wednesday afternoon, 3pm.
   
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