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Team:Newcastle/Meetings/26 July 2010
From 2010.igem.org
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==Research feedback== | ==Research feedback== | ||
| - | #Levan glue - Levan requires sucrose to work | + | # Levan glue - Levan requires sucrose to work |
* Test how much sucrose is needed - Phil | * Test how much sucrose is needed - Phil | ||
| - | #Kill switch - Use of ''mazE'' and ''mazF'' | + | # Kill switch - Use of ''mazE'' and ''mazF'' |
* How much sucrose is needed for it to work? | * How much sucrose is needed for it to work? | ||
* How fast does the bug use up all the sucrose? | * How fast does the bug use up all the sucrose? | ||
| - | #Stochastic switch - Use of ''hinC'' as the invertase | + | # Stochastic switch - Use of ''hinC'' as the invertase |
* Matt to work with Phil to work out the probability of the switching | * Matt to work with Phil to work out the probability of the switching | ||
* Have to work out what is wrong with the iGEM 2009 stochastic switch first | * Have to work out what is wrong with the iGEM 2009 stochastic switch first | ||
| - | #Gibson protocol | + | # Gibson protocol |
* ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first | * ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first | ||
==Lab feedback== | ==Lab feedback== | ||
| - | #Arginine test - Alan and Harsh | + | # Arginine test - Alan and Harsh |
* Test worked fine. | * Test worked fine. | ||
* Could refine the test by using universal liquid pH indicator or to use the pH meter. | * Could refine the test by using universal liquid pH indicator or to use the pH meter. | ||
| - | #DNA extraction - Harsh and Racheal | + | # DNA extraction - Harsh and Racheal |
* Could only confirm the result on 27.07.2010. | * Could only confirm the result on 27.07.2010. | ||
Revision as of 14:24, 27 July 2010
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Contents |
Roll call
Everyone here except Colin Harwood, Colin Davie and Wendy Smith
Research feedback
- Levan glue - Levan requires sucrose to work
- Test how much sucrose is needed - Phil
- Kill switch - Use of mazE and mazF
- How much sucrose is needed for it to work?
- How fast does the bug use up all the sucrose?
- Stochastic switch - Use of hinC as the invertase
- Matt to work with Phil to work out the probability of the switching
- Have to work out what is wrong with the iGEM 2009 stochastic switch first
- Gibson protocol
- rocF to require 6 pair of primers, therefore try doing up 2 fragments first
Lab feedback
- Arginine test - Alan and Harsh
- Test worked fine.
- Could refine the test by using universal liquid pH indicator or to use the pH meter.
- DNA extraction - Harsh and Racheal
- Could only confirm the result on 27.07.2010.
Logo and T-shirts
- Have all logos on t-shirt
- Bacilla filla words on top of logo
Visas
- Harsh to travel to London on 160710.
Travel plans
- Book flights from 041110 to the 081110
- Accommodation open for booking
Funding
- School of Biomedical Sciences
- Mr. Gene might be able to fund us because we display their logo on wiki
Other business
- If start later, email everyone first.
Action points
- Write thank you letter to David Parker
- CEG logo on website
- Talk to Lula about funding
- Get back to Centre of Life person
- Contact Megan Lazara for iGEM badges and stickers
- Phil to send an email to Jill Green about Get Together
- Legends on figures(table caption goes on top, picture captions bottom), write on, description
- Create a page for cloning strategy like last year's
- Twitter feed
- Jannetta to change the gliffs
- What to do with yneA when it comes back
- Plan every step before going into lab
- Fill in wiki everyday for lab work
- Put down where we get protocols from
- Have a protocol page and link all experiments to that page
- Put Jane Calvert on iGEM mailing list
- Put all school logos on website
- Fill immigration form about 2 weeks before jamboree
Next meeting
- Steven is chair, Deena is computer, Alan is minutes
- Friday afternoon, 4pm.
   
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