Team:Newcastle/27 July 2010

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(Genomic DNA extraction experiment)
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===Genomic DNA extraction experiment===
===Genomic DNA extraction experiment===
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====Aim====
====Aim====
The aim of today's experiment is to extract genomic DNA from ''Bacillus subtilis'' strain 3610 and 168,genes from which will be needed for the swarming biobrick and ''rocF'' biobrick.  
The aim of today's experiment is to extract genomic DNA from ''Bacillus subtilis'' strain 3610 and 168,genes from which will be needed for the swarming biobrick and ''rocF'' biobrick.  

Revision as of 13:52, 27 July 2010

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Contents

Genomic DNA extraction experiment

P7270475.JPG

Aim

The aim of today's experiment is to extract genomic DNA from Bacillus subtilis strain 3610 and 168,genes from which will be needed for the swarming biobrick and rocF biobrick.

Procedure

Discussion

At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.

Conclusion

Th experiment was successful and we would check the content and the purity of the extracted DNA by using PCR on 28th July, 2010.

Gel Electrophoresis

P7270470.JPG

Materials

  1. 1% Agarose TAE
  2. SafeView
  3. TAE Buffer

Protocol

Discussion

  • No band was observed on the gel.

Conclusion

The PCR did not work. It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.


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