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Team:EPF Lausanne/Calendar/0720
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| - | + | *Plating Asaia to get WT (once again) | |
| + | *Making HEPES Buffer for the Competence of Asaia | ||
| + | *Diluted the culture to get a 25ml culture(1:20) according to Asaia competence protocol | ||
| + | *OD curve plotted, Doubling time calculated | ||
| + | *No single colonies from the re-growing trial --> replating properly on separate plates | ||
| + | *Preparation of LB medium mixed with Kan/Amp/Cm and GLY medium mixed with <kan | ||
| + | *Miniprep of Origin + Resistance | ||
| + | *Miniprep of Origin alone. | ||
| + | *Miniprep of base vector BBa_151020 | ||
| + | *Concentration measurments of miniprep products | ||
| + | *Glycerol stock of BB plasmid and Base Vector made | ||
</div> | </div> | ||
Latest revision as of 18:53, 23 July 2010
20 July 2010
- Plating Asaia to get WT (once again)
- Making HEPES Buffer for the Competence of Asaia
- Diluted the culture to get a 25ml culture(1:20) according to Asaia competence protocol
- OD curve plotted, Doubling time calculated
- No single colonies from the re-growing trial --> replating properly on separate plates
- Preparation of LB medium mixed with Kan/Amp/Cm and GLY medium mixed with <kan
- Miniprep of Origin + Resistance
- Miniprep of Origin alone.
- Miniprep of base vector BBa_151020
- Concentration measurments of miniprep products
- Glycerol stock of BB plasmid and Base Vector made
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