Team:Caltech/Week 5

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Revision as of 00:27, 14 July 2010

iGEM 2010

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Group meeting today!
Sequencing results were really crappy (quality < 30% confidence), so began 2 5mL LB cultures of the light-lysis construct (picked from the original transformation plate).

Sent off two new samples of the light-lysis construct for sequencing using the VF2 and VR primers. Results should be available tonight or tomorrow.
Used remaining cells to inoculate new cultures of each and to extract DNA for a test digest:
- Digested each (ideally identical) sample with EcoRI-HF and PstI, both for 1hr and for 10min.
- The digest appears inconclusive, as a smear is visible from the top to about 3Kb, while little or no DNA is visible in the insert range (~1.5Kb, see below).

Gel image for the light-lysis test digest. (7/13)

- Will run another gel tonight with other bricks to check efficacy of digests and will look forward to sequencing results soon.

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@@ Line 11: / Line 11: @@
 * Used remaining cells to inoculate new cultures of each and to extract DNA for a test digest:
 ** Digested each (ideally identical) sample with EcoRI-HF and PstI, both for 1hr and for 10min.
-** The digest appears inconclusive, as a smear is visible from the top to about 3Kb, while little or no DNA is visible in the insert range (~1.5Kb).
+** The digest appears inconclusive, as a smear is visible from the top to about 3Kb, while little or no DNA is visible in the insert range (~1.5Kb, see below).
+[[Image:PRlyTgel7-13.jpg|200px|thumb|right|Gel image for the light-lysis test digest. (7/13)]]
 ** Will run another gel tonight with other bricks to check efficacy of digests and will look forward to sequencing results soon.