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Team:METU Turkey/Planning
From 2010.igem.org
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| - | <br>Design and Synthesis of Response Elements | + | <br> |
| + | <br>Cloning | ||
| + | <br> - Design and Synthesis of Response Elements | ||
<br> - Construction of Cell Sensor modules | <br> - Construction of Cell Sensor modules | ||
<br> - Preparation of competent cell | <br> - Preparation of competent cell | ||
Revision as of 03:28, 28 October 2010
PROJECT EXECUTION
Cloning
- Design and Synthesis of Response Elements
- Construction of Cell Sensor modules
- Preparation of competent cell
- Design and ordering of DNA parts
- Cloning
- Validation
- Assembly of final construct
Cell Sensor Experiments
- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy
CooA Expression and Purification
- Overexpression optimization
- Purification optimization
Characterization: Isothermal Titration Calorimetry (ITC)
- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
Characterization: Electrophoretic Mobility Shift Assay (EMSA)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
Characterization: Intrinsic Tryptophan Fluorescence (ITF)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
