Team:Hong Kong-CUHK/Parts
From 2010.igem.org
(Difference between revisions)
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</li></ul> | </li></ul> | ||
<p>About 15-20 % inversion / recombination | <p>About 15-20 % inversion / recombination | ||
- | </p><p> | + | </p><p></p> |
- | </p> | + | |
<ul><li>Reference: | <ul><li>Reference: | ||
</li></ul> | </li></ul> | ||
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<p> </p> | <p> </p> | ||
- | <p>< | + | <p><h3>Message gene template</h3></p> |
- | <p>This message system consists of a | + | <p>This message system consists of a message sequence, with specific repeat sequences inserted as to be recognized by the Rci site-specific recombinase.</p> |
<ul> | <ul> | ||
- | <li>This message has 70 characters | + | <li>This message has 70 characters - "We must learn to live together as brothers or perish together as tools"</li> |
</ul> | </ul> | ||
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<ul> | <ul> | ||
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<p>In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.</p> | <p>In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.</p> | ||
- | + | <p><img alt="Image:msg.png" src="http://partsregistry.org/wiki/images/9/91/Msg.png" width="600" height="178" border="0"> | |
+ | </p> | ||
Reference:<br /> | Reference:<br /> | ||
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<p>Gyohda, A. & Funayama, N. (1997). <em>Analysis of DNA Inversions in the Shufflon of Plasmid R64</em>. American Society of Microbiology, 179 (6), 1867-1871.</p> | <p>Gyohda, A. & Funayama, N. (1997). <em>Analysis of DNA Inversions in the Shufflon of Plasmid R64</em>. American Society of Microbiology, 179 (6), 1867-1871.</p> | ||
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<p> </p> | <p> </p> | ||
- | <p>< | + | <p><h3>Rci recombinase system</h3></p> |
<p>This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.</p> | <p>This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.</p> |
Latest revision as of 03:08, 28 October 2010