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Team:Chiba/System 2/Result
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===1.Construction Plux inv-GFP=== | ===1.Construction Plux inv-GFP=== | ||
| - | Construction process is shown in '''Fig. 1'''.Plux inv-GFP | + | Construction process is shown in '''Fig. 1'''.We constructed Plux inv-GFP conbining R0061 and E0240 and transformed by strain of XL10-G. |
| - | + | GFP fluorescence and sequence is confirmed. | |
[[Image:Plux inv-GFP.png|none|frame|right|'''Fig. 1''' Evaluation of LuxR Inverter]] | [[Image:Plux inv-GFP.png|none|frame|right|'''Fig. 1''' Evaluation of LuxR Inverter]] | ||
Revision as of 23:56, 27 October 2010
Version 2 :
Contents |
Abstract
LuxR is AHL-dependent activator.LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription.However lux box is inserted between -35 and -10,LuxR functions as AHL-inverter (Plux inv). Plux inv is resistered in Biobrick number R0061.We've prepared Plux inv-GFP and characterized about it.
Experiments
1.Construction Plux inv-GFP
Construction process is shown in Fig. 1.We constructed Plux inv-GFP conbining R0061 and E0240 and transformed by strain of XL10-G.
GFP fluorescence and sequence is confirmed.
Fig. 1 Evaluation of LuxR Inverter
2.Characterization Plux inv-GFP
Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。コロニーをつついてLB液体培地(AHL0および1000 nM)で12 h培養し, スピンダウンしてペレットを観察した。
Fig. 2 assay protocol
Results
Reference
- Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
- Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)
