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Team:Edinburgh/Notebook/Green light sensor
From 2010.igem.org
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[[Image:148.JPG]] | [[Image:148.JPG]] | ||
| + | '''19/10/20010''' | ||
| + | * Lane 1: marker | ||
| + | * Lane 2: PheA (very faint band or primer smear?) | ||
| + | * Lane 3: Ccas + PhoR ligation | ||
| + | |||
| + | [[Image:173.JPG]] | ||
| + | |||
| + | '''22/10/2010''' | ||
| + | * PhoA + LacZ | ||
| + | |||
| + | [[Image:174.JPG]] | ||
| + | |||
| + | '''23/10/2010''' | ||
| + | * Lane 1: marker | ||
| + | * Lane 2: phA promoter + lac Z | ||
| + | * rest- irrelevant | ||
| + | |||
| + | [[Image:175.JPG]] | ||
| + | |||
| + | '''25/10/2010''' | ||
| + | * pCB + green light sensor | ||
| + | |||
| + | [[Image:176.JPG]] | ||
| + | |||
| + | '''26/10/2010''' | ||
| + | * total green light sensor + reporter | ||
| + | |||
| + | [[Image:177.JPG]] | ||
| + | |||
| + | '''27/10/2010''' | ||
| + | * green light sensor ligations into PSB1C3 | ||
| + | |||
| + | [[Image:178.JPG]] | ||
<br> | <br> | ||
Revision as of 22:03, 27 October 2010
These notes correspond to the part of the project detailed here.
Green Light Sensor
8/10/2010
- running purified PCR: marker (lane 1), PhOR (Lane 2), Ccas (Lane 3).
19/10/20010
- Lane 1: marker
- Lane 2: PheA (very faint band or primer smear?)
- Lane 3: Ccas + PhoR ligation
22/10/2010
- PhoA + LacZ
23/10/2010
- Lane 1: marker
- Lane 2: phA promoter + lac Z
- rest- irrelevant
25/10/2010
- pCB + green light sensor
26/10/2010
- total green light sensor + reporter
27/10/2010
- green light sensor ligations into PSB1C3
