Team:Edinburgh/Notebook/Green light sensor

From 2010.igem.org





These notes correspond to the part of the project detailed here.

Green Light Sensor



8/10/2010

  • running purified PCR: marker (lane 1), PhOR (Lane 2), Ccas (Lane 3).

Image:148.JPG

19/10/20010

  • Lane 1: marker
  • Lane 2: PheA (very faint band or primer smear?)
  • Lane 3: Ccas + PhoR ligation

Image:173.JPG

22/10/2010

  • PhoA + LacZ

Image:174.JPG

23/10/2010

  • Lane 1: marker
  • Lane 2: phA promoter + lac Z
  • rest- irrelevant

Image:175.JPG

25/10/2010

  • pCB + green light sensor

Image:176.JPG

26/10/2010

  • total green light sensor + reporter

Image:177.JPG

27/10/2010

  • green light sensor ligations into PSB1C3

Image:178.JPG


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