Team:METU Turkey/Results Discussion/CooA Expression and Purification

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<br> A. Expression vector comparison (pKK versus pTriEx)
<br> A. Expression vector comparison (pKK versus pTriEx)
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<br> A.1. Expression in BL21 with pTriEx
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<br> A.2. Expression in JM109 and BL21 with pKK vector
<br>  <html><div><a href="https://2010.igem.org/Team:METU_Turkey/Results_Discussion/pKK_versus_pTriEx"><strong>Details...</strong></a></div></html>
<br>  <html><div><a href="https://2010.igem.org/Team:METU_Turkey/Results_Discussion/pKK_versus_pTriEx"><strong>Details...</strong></a></div></html>
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<br> A. Ion exchange chromatography with Sepharose Q (QS)
<br> A. Ion exchange chromatography with Sepharose Q (QS)
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Revision as of 19:06, 27 October 2010

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3-CooA Expression and Purification



1-Overexpression optimization
A. Expression vector comparison (pKK versus pTriEx)
A.1. Expression in BL21 with pTriEx
A.2. Expression in JM109 and BL21 with pKK vector



B. Expression host comparison (JM109 versus BL21)



C. IPTG induction studies


D. Ferric Citrate effect


E. Soluble versus insoluble fraction


2-Purification optimization


A. Ion exchange chromatography with Sepharose Q (QS)





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