Team:METU Turkey/Planing

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<li><a href="https://2010.igem.org/Team:METU_Turkey/Links">LINKS</a></li>
<li><a href="https://2010.igem.org/Team:METU_Turkey/Links">LINKS</a></li>
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<br>
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<br> PROJECT EXECUTION
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<br>
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<br>
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<br> Design and Synthesis of Response Elements
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<br>
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<br> - Construction of Cell Sensor modules
 +
<br> - Preparation of competent cell
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<br> - Design and ordering of DNA parts
 +
<br> - Cloning
 +
<br> - Validation
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<br> - Assembly of final construct
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<br>
 +
<br>
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<br> Cell Sensor Experiments
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<br> - Fermentor studies
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<br> - Flask experiments
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<br> - Solid culture experiments
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<br> - Myoglobin assay optimization
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<br> - Signal quantification with fluorescence spectroscopy
 +
<br> - Imaging with confocal laser scanning microscopy
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<br>
 +
<br>
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<br> CooA Expression and Purification
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<br> - Overexpression optimization
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<br> - Purification optimization
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<br>
 +
<br>
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<br>Characterization: Isothermal Titration Calorimetry (ITC)
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<br> - DNA component preparation
 +
<br> - Optimization studies
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<br> - Determination of Response Element - Transcription Factor Affinities
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<br>
 +
<br>
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<br>Characterization: Electrophoretic Mobility Shift Assay (EMSA)
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<br>- Optimization studies
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<br>- Determination of Response Element - Transcription Factor Affinities
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<br>
 +
<br>
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<br>Characterization: Intrinsic Tryptophan Fluorescence (ITF)
 +
<br> - Optimization studies
 +
<br> - Determination of Response Element - Transcription Factor Affinities

Latest revision as of 18:06, 27 October 2010

Home

 




PROJECT EXECUTION


Design and Synthesis of Response Elements

- Construction of Cell Sensor modules
- Preparation of competent cell
- Design and ordering of DNA parts
- Cloning
- Validation
- Assembly of final construct


Cell Sensor Experiments
- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy


CooA Expression and Purification
- Overexpression optimization
- Purification optimization


Characterization: Isothermal Titration Calorimetry (ITC)
- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities


Characterization: Electrophoretic Mobility Shift Assay (EMSA)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities


Characterization: Intrinsic Tryptophan Fluorescence (ITF)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

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