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Team:Imperial College London/Modelling/Signalling/Results and Conclusion
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If we change either [AIP]<html><sub>0</sub></html> or [ComE]<html><sub>0</sub></html>, then the final concentration of ComE* will always tend towards <html>5×10<sup>-11</sup>M</html>. <html>[ComE*]<sub>final</sub></html> will always tend towards this value, unless the initial concentrations of Phosphate and ComE are changed. However, if we increase both [AIP]<html><sub>0</sub></html> and [ComE]<html><sub>0</sub></html> at the same time, then [ComE*]<html><sub>final</sub></html> will be reached much faster (i.e. slope increases). | If we change either [AIP]<html><sub>0</sub></html> or [ComE]<html><sub>0</sub></html>, then the final concentration of ComE* will always tend towards <html>5×10<sup>-11</sup>M</html>. <html>[ComE*]<sub>final</sub></html> will always tend towards this value, unless the initial concentrations of Phosphate and ComE are changed. However, if we increase both [AIP]<html><sub>0</sub></html> and [ComE]<html><sub>0</sub></html> at the same time, then [ComE*]<html><sub>final</sub></html> will be reached much faster (i.e. slope increases). | ||
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| - | |''' | + | |'''Equation:''' AIP-ComD*-ComE <html>↔</html> AIP-ComD + ComE* |
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| - | |[[Image:IC_Signalling_Results2.png| | + | |[[Image:IC_Signalling_Results2.png|270px]] [[Image:IC_Signalling_Results3.png|270px]] [[Image:IC_Signalling_Results4.png|260px]] |
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| - | |Graph showing the production of Pr-ComD complex. | + | |'''1.''' Graph showing the production of Pr-ComD complex. (Equation 1: AIP + ComD <html>↔</html> AIP-ComD). |
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| - | |''' | + | |'''2.''' Graph showing the production of phosphorylated Pr-ComD* complex. (Equation 2: AIP-ComD + Phosphate <html>↔</html> AIP-ComD*). |
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| - | |''' | + | |'''3.''' Graph showing the production of Pr-ComD*-ComE complex. (Equation 3: AIP-ComD* + ComE <html>↔</html> AIP-ComD*-ComE. |
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| - | |< | + | |Notice the steep increase of concentration for each of the graphs, which could be due to high k<html><sub>1,2,3</sub></html> values. |
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Revision as of 15:25, 27 October 2010
| Modelling | Overview | Detection Model | Signaling Model | Fast Response Model | Interactions |
| A major part of the project consisted of modelling each module. This enabled us to decide which ideas we should implement. Look at the Fast Response page for a great example of how modelling has made a major impact on our design! | |
| Objectives | Description | Results | Constants | MATLAB Code |
| Results and Conclusion | |||||
| Using this model, we can show that the phosphorylated ComE* is proportional to both initial concentration of AIP and ComD.
If the initial concentration of AIP or ComD is zero, there is no formation of ComE*. We are assuming an initial concentration of Phosphate and ComE of 100nM. If we change either [AIP]0 or [ComE]0, then the final concentration of ComE* will always tend towards 5×10-11M. [ComE*]final will always tend towards this value, unless the initial concentrations of Phosphate and ComE are changed. However, if we increase both [AIP]0 and [ComE]0 at the same time, then [ComE*]final will be reached much faster (i.e. slope increases). | |||||
| Equation: AIP-ComD*-ComE ↔ AIP-ComD + ComE* | |||||
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