"
Team:Tsinghua/Notebook/8 October 2010
From 2010.igem.org
(Difference between revisions)
(→Module I, Group 2c) |
Lonelyroads (Talk | contribs) (→Module I, Group 2c) |
||
| Line 1: | Line 1: | ||
| + | == Module I, Group 1A == | ||
| + | Extract plasmid ptv. Transform into competent with pAH69 through electrotransformation. Spread to 0.03% tetracyclin plates. | ||
| + | |||
== Module I, Group 2c == | == Module I, Group 2c == | ||
Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. | Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. | ||
Latest revision as of 15:01, 27 October 2010
Module I, Group 1A
Extract plasmid ptv. Transform into competent with pAH69 through electrotransformation. Spread to 0.03% tetracyclin plates.
Module I, Group 2c
Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I.
Double digestion system
eGFP 5ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 11ul
mCherry 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Kan 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Incubate at 37 centidegree overnight.
