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Team:Warsaw/Calendar-Stage1/1 July 2010
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| - | + | <h3><div style="text-align: center;">Main topic</div></h3> | |
| + | <h4>Marcin</h4> | ||
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| + | Task 1: Prepare bacterial culture to isolate <a href="http://partsregistry.org/Part:BBa_K177044" style="color: black">BBa_K177044</a>] from [http://partsregistry.org/Part:pSB1A3<span style="color:black">pSB1A3</span>]. | ||
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| + | Task 2: Another ligation of [http://partsregistry.org/Part:BBa_K177044<span style="color: black">BBa_K177044</span>] | ||
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| + | Methods: | ||
| + | * Ligation mixtures composition: | ||
| + | <pre> | ||
| + | 10 μl digested insert | ||
| + | 8 μl digested vector | ||
| + | 5 μl Tango buffer(Fermentas) | ||
| + | 2 μl ATP 10 mM solution | ||
| + | 4 μl MQ water | ||
| + | 1 μl ligase T4 (Fermentas) | ||
| + | </pre> | ||
| + | * Ligation was carried out about 20 hours and subsequently inactivated | ||
| + | |||
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Revision as of 21:19, 9 July 2010
Main topic
Marcin
Task 1: Prepare bacterial culture to isolate BBa_K177044] from [http://partsregistry.org/Part:pSB1A3pSB1A3]. Task 2: Another ligation of [http://partsregistry.org/Part:BBa_K177044BBa_K177044] Methods: * Ligation mixtures composition:10 μl digested insert 8 μl digested vector 5 μl Tango buffer(Fermentas) 2 μl ATP 10 mM solution 4 μl MQ water 1 μl ligase T4 (Fermentas)* Ligation was carried out about 20 hours and subsequently inactivated
