Team:Calgary/19 September 2010
From 2010.igem.org
Line 9: | Line 9: | ||
Today, I did plasmid preparations of the CpxP and ibpAB-fsxA fusion promoter overnight cultures that were set up yesterday. The concentrations are available on the tubes. Some of the promoters were set up in AK and AC in order to facilitate future construction. Then, I set up restriction digests using XbaI and PstI to be run on a gel as the final step in verification. I also set up a DNA PCR of the CpxP and ibpAB which will be run on a gel tomorrow. | Today, I did plasmid preparations of the CpxP and ibpAB-fsxA fusion promoter overnight cultures that were set up yesterday. The concentrations are available on the tubes. Some of the promoters were set up in AK and AC in order to facilitate future construction. Then, I set up restriction digests using XbaI and PstI to be run on a gel as the final step in verification. I also set up a DNA PCR of the CpxP and ibpAB which will be run on a gel tomorrow. | ||
+ | |||
+ | <u>Dev</u> | ||
+ | |||
+ | The construction of the CpxR promoter with I13504 and the CpxR promoter with I13507 failed and therefore was attempted again. This time 25µL of both insert and vector were ligated together instead of 5µL. The transformation procedure remained the same. | ||
+ | |||
}} | }} |
Latest revision as of 01:36, 27 October 2010
![University of Calgary iGEM Team 2010](http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Header4.png)
Sunday September 19, 2010
Weekends? Who needs those?
Chris
Today, I did plasmid preparations of the CpxP and ibpAB-fsxA fusion promoter overnight cultures that were set up yesterday. The concentrations are available on the tubes. Some of the promoters were set up in AK and AC in order to facilitate future construction. Then, I set up restriction digests using XbaI and PstI to be run on a gel as the final step in verification. I also set up a DNA PCR of the CpxP and ibpAB which will be run on a gel tomorrow.
Dev
The construction of the CpxR promoter with I13504 and the CpxR promoter with I13507 failed and therefore was attempted again. This time 25µL of both insert and vector were ligated together instead of 5µL. The transformation procedure remained the same.