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Team:UPO-Sevilla/Notebook/09 14
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<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
| - | <p>Minipreps of (1+2) and (16+3) to get the plasmids. Digestion of the first one with the restriction enzymes SpeI and PstI, and the second one with XbaI and PstI, that is to say, using standard assembly We run an electrophoresis to isolate and purify the suitable fragments. We ligate them o/n.</p> | + | <p>Minipreps of (1+2) and (16+3) to get the plasmids. Digestion of the first one with the restriction enzymes SpeI and PstI, and the second one with XbaI and PstI, that is to say, using standard assembly. We run an electrophoresis to isolate and purify the suitable fragments. We ligate them o/n.</p> |
<h2>Assay Team</h2> | <h2>Assay Team</h2> | ||
| - | <p>We | + | <p>We developed different assays by changing different features; the plates were incubated overnight.</p> |
Latest revision as of 21:02, 26 October 2010
September, 14th
Assembly Team
Minipreps of (1+2) and (16+3) to get the plasmids. Digestion of the first one with the restriction enzymes SpeI and PstI, and the second one with XbaI and PstI, that is to say, using standard assembly. We run an electrophoresis to isolate and purify the suitable fragments. We ligate them o/n.
Assay Team
We developed different assays by changing different features; the plates were incubated overnight.
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