Team:UPO-Sevilla/Notebook/09 14


September, 14th

Assembly Team

Minipreps of (1+2) and (16+3) to get the plasmids. Digestion of the first one with the restriction enzymes SpeI and PstI, and the second one with XbaI and PstI, that is to say, using standard assembly. We run an electrophoresis to isolate and purify the suitable fragments. We ligate them o/n.

Assay Team

We developed different assays by changing different features; the plates were incubated overnight.

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