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Team:TU Delft/21 June 2010 content
From 2010.igem.org
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===Lab work=== | ===Lab work=== | ||
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| + | [[Team:TU_Delft/protocols/restriction_enzyme_digestion|Restriction digestion]] of plasmid backbone pSB1T3: | ||
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|pSB1T3 (~1,0 μg) | |pSB1T3 (~1,0 μg) | ||
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Cut digested pSB1T3 from gel and isolate the band. | Cut digested pSB1T3 from gel and isolate the band. | ||
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| + | We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day. | ||
Revision as of 08:46, 6 July 2010
Lab work
Restriction digestion of plasmid backbone pSB1T3:
| pSB1T3 (~1,0 μg) | 2,5 μL |
| Buffer H (Roche) | 2,0 μL |
| EcoRI (1 U/μg) | 1,0 μL |
| PstI (1 U/μg) | 1,0 μL |
| H2O | 13,5 μL |
Cut digested pSB1T3 from gel and isolate the band.
We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
