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From 2010.igem.org

Lab work

Characterization of Anderson RBS sequences

Restriction digestion of plasmid backbone pSB1T3.

#	Sample	Enzyme 1	Enzyme 2	Buffer	BSA	Needed fragment
1	1.0 μg pSB1T3	EcoRI	PstI	H (Roche)	✗	‘E–linear pSB1T3–P’

In stead of 10 U/μL restriction enzyme we used 1 U/μL. The digested pSB1T3 was cut from gel and isolate the band. However, the gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.