Team:KIT-Kyoto/Parts

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Revision as of 07:02, 26 October 2010

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We used these parts for making our original biobrick parts

1.We obtained these parts from 2010 iGEM kit

Name	Part type	Resistance	Insert	Vector	Contents
<partinfo>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>)	Plasmid backbone	Kanamycin	738bp	2750bp	promoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_J04450</partinfo>)	Plasmid backbone	Ampicillin	738bp	4032bp	promoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1C3</partinfo>(<partinfo>BBa_J04450</partinfo>)	Plasmid backbone	Chloramphenicol	1069bp	2072bp	promoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I3522</partinfo>)	Composite	Ampicillin	937bp	2079bp	promoter(TetR)+RBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_R0040</partinfo>)	Regulatory	Ampicillin	54bp	2079bp	promoter(TetR)
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I732019</partinfo>)	Regulatory	Ampicillin	3230bp	2079bp	RBS+LacZ+T+T
<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)	Protein domain	Kanamycin	3230bp	2079bp	RBS+LacZ+T+T
<partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>)	Composite	Ampicillin	1896bp	3395bp
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>)	Protein domain	Ampicillin	883bp	2079bp	RBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13507</partinfo>)	Protein domain	Ampicillin	937bp	2079bp	RBS+RFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0420</partinfo>)	Protein domain	Ampicillin	878bp	2079bp	RBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0430</partinfo>)	Protein domain	Ampicillin	878bp	2079bp	RBS+YFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13600</partinfo>)	Composite	Ampicillin	940bp	2079bp	promoter(TetR)+RBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_J22005</partinfo>)	Composite	Ampicillin	2079bp	2623bp	promoter(TetR)+RBS+YFP+T+T

2.We obtained this part directly from iGEM HQ

We characterize this BioBrick Part and enter the new information back on the Registry. This parts don’t include detailed information on pSB6 and the reasons leading to its usage in our work to produce GFP. In general, pSB6 is a low copy plasmid which makes it more suitable for protein production than the high copy vector. However this information cannot be found in any sites related to iGEM. Consequently, we tried to compare the performances of the high copy vector pSB1 and low copy vector pSB6. We have confirmed in this way that pSB6 is more efficient at producing GFP protein than PSB1. We have thus proved that this part is very useful to evaluate the capabilities of a promoter, in this case by observing the degree of fluorescence of GFP protein. We are very thankful to the 09’Tokyo-Tech group who has supplied us with this part.
>>About pSB1 vs pSB6

Name	Part Type	Resistance	Insert	Vector
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_K121013</partinfo>)	Protein domain	Ampicillin	883bp	4022bp	RBS+GFP+T+T

We designed and constructed these parts originally.

We cloned the following inserts into the vector, pSB6A1. Out of them we cloned the two inserts(BBa_K362005, BBa_K362007) into the pSB1C3 to send to the iGEM headquarter.

<groupparts>iGEM010 KIT-Kyoto</groupparts>

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-== We make or design these parts originally. ==
+== We designed and constructed these parts originally. ==
-We transformed these inserts into the vector,pSB6A1.And we transformed 2 inserts(BBa_K362005,BBa_K362007) of them into pSB1C3.
+We cloned the following inserts into the vector, pSB6A1. Out of them we cloned the two inserts(BBa_K362005, BBa_K362007) into the pSB1C3 to send to the iGEM headquarter.
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