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Team:Newcastle/Meetings/4 June 2010
From 2010.igem.org
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| + | ==Informal Meeting - 04.06.2010== | ||
| + | ====Urease tutorial-recap==== | ||
| + | * Accessory genes and nickel incorporation necessary more complicated than we thought. | ||
| + | * Software engineering cycle | ||
| + | * Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements | ||
| + | * Specification more detail on the requirements- literature | ||
| + | * Urease calcium carbonate, optimise for Bacillis | ||
| + | * Increase expression- what we thought! | ||
| + | * Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper | ||
| + | * Accessory genes required! | ||
| + | * Look over papers from tutorial | ||
| + | * Sporosarcina pasteurii | ||
| + | * Biobrick site symbols | ||
| + | * Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers | ||
| + | * pSpac | ||
| + | * ncbi – find the genome – can see the genes | ||
| + | * Implementation using sequencher | ||
| + | * Find the sequence of part | ||
| + | * Assemble parts | ||
| + | * Biobrick friendly restriction sites | ||
| + | * Genbank/embl file format | ||
| + | * Implementation altered by cloning strategy | ||
| + | * Biobricks RFC- request for comments | ||
| + | * http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts -duplo and lego don’t fit together | ||
| + | * AP Rachel: video for lego pipette:http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded | ||
| - | + | ====1st lab week==== (Others modelling Filamentous and spidersilk killswitch) | |
| + | * RC | ||
| + | * DY | ||
| + | * AK | ||
| + | * (HS) | ||
| + | |||
| + | ====2nd lab week==== (Others Modelling glue and lysis quorum sensing) | ||
| + | *JS | ||
| + | *RB | ||
| + | *SW | ||
| + | *PH | ||
| + | *(YE) | ||
| + | *Urease both | ||
| + | |||
| + | ====LAB TRAINING GROUPS:==== | ||
* '''Group 1 starts 14th June''': SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis) | * '''Group 1 starts 14th June''': SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis) | ||
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* Team members not in the lab training will be working on modeling. | * Team members not in the lab training will be working on modeling. | ||
* HS and YE will unfortunately be away for both lab training sessions, hence the brackets | * HS and YE will unfortunately be away for both lab training sessions, hence the brackets | ||
| + | |||
| + | {{Team:Newcastle/footer}} | ||
Revision as of 03:05, 26 October 2010
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Informal Meeting - 04.06.2010
Urease tutorial-recap
- Accessory genes and nickel incorporation necessary more complicated than we thought.
- Software engineering cycle
- Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
- Specification more detail on the requirements- literature
- Urease calcium carbonate, optimise for Bacillis
- Increase expression- what we thought!
- Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
- Accessory genes required!
- Look over papers from tutorial
- Sporosarcina pasteurii
- Biobrick site symbols
- Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
- pSpac
- ncbi – find the genome – can see the genes
- Implementation using sequencher
- Find the sequence of part
- Assemble parts
- Biobrick friendly restriction sites
- Genbank/embl file format
- Implementation altered by cloning strategy
- Biobricks RFC- request for comments
- http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts -duplo and lego don’t fit together
- AP Rachel: video for lego pipette:http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded
====1st lab week==== (Others modelling Filamentous and spidersilk killswitch)
- RC
- DY
- AK
- (HS)
====2nd lab week==== (Others Modelling glue and lysis quorum sensing)
- JS
- RB
- SW
- PH
- (YE)
- Urease both
LAB TRAINING GROUPS:
- Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)
- Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)
- All team members working on BioBrick 1 (CaCO3/Urease).
- RC working on BioBrick 6 (sequential programming)
- Team members not in the lab training will be working on modeling.
- HS and YE will unfortunately be away for both lab training sessions, hence the brackets
   
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