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Team:Newcastle/20 August 2010
From 2010.igem.org
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| - | ==Transformation of | + | ==Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3== |
| - | + | ==Aims== | |
| - | To produce more colonies of the | + | To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing ''yneA'' with pGFPrrnB and pSB1C3 from[[Team:Newcastle/19_August_2010|yesterday]]. |
| - | + | ==Materials and Protocol== | |
Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]]. | Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]]. | ||
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==Ligation== | ==Ligation== | ||
Revision as of 02:45, 26 October 2010
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Contents |
Miniprep for yneA, pGFPrrnB and pSB1C3
Aim
The aim of this experiment is to produce stocks of yneA, pGFPrrnB and pSB1C3.
Materials and Protocol
Please refer to miniprep and nanodrop.
Results
| Sample no. | yneA | pGFPrrnB | pSB1C3 |
|---|---|---|---|
| 1 | 432.5 | 238.9 | 126.1 |
| 2 | 347.6 | 230.7 | 107.6 |
| 3 | 380.2 | 390.9 | 121.5 |
| 4 | 377.9 | 236.2 | 112.8 |
Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.
Discussion
Results from the NanoDrop show concentration values of more than 100 ng/µl, which means we have obtained good concentration of DNA from the miniprep.
Conclusion
We keep the miniprep products as stocks for future use.
Transformation of ligated yneA into pGFPrrnB and pSB1C3
Aims
To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.
Materials and Protocol
Please refer to transformation of E. coli.
Ligation
Aims
To ligate yneA with pGFPrrnB and yneA with pSB1C3 (A repeat of yesterday).
Materials and Protocol
Please refer to ligation.
Results, Discussion and Conclusion
Please refer to 23.08.10.
Plating Bacillus subtilis BFS678
Has pMutin4 (and thus lacI) integrated into the chromosome...will be useful for characterisation.. will be transforming this strain with our filamentous cell and arginase BioBricks.
   
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