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Team:Newcastle/Meetings/6 October 2010
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| - | =6 October 2010 | + | ==Formal Meeting - 6 October 2010== |
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* Apologies: Colin Harwood, Jem and Phil | * Apologies: Colin Harwood, Jem and Phil | ||
| - | ==Presentation== | + | ===Presentation=== |
| - | ===Introduction=== | + | ====Introduction==== |
* Mention why do we use this bug and that it is able to live in high pH | * Mention why do we use this bug and that it is able to live in high pH | ||
* Have to menetion that we all biobrick have been modelled by SBML and copasi | * Have to menetion that we all biobrick have been modelled by SBML and copasi | ||
| - | ===Bacilla Filla slide=== | + | ====Bacilla Filla slide==== |
* The solution words to be smaller than the logo | * The solution words to be smaller than the logo | ||
* Logo have to be the biggest! | * Logo have to be the biggest! | ||
* Solution at the top of the page with a small logo below it then zoom into the logo | * Solution at the top of the page with a small logo below it then zoom into the logo | ||
| - | ===Technical review slide=== | + | ====Technical review slide==== |
* Show the link between the genes | * Show the link between the genes | ||
* Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc. | * Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc. | ||
| Line 26: | Line 24: | ||
* Animation for the overview, ie, the background to remain the same | * Animation for the overview, ie, the background to remain the same | ||
| - | ===Swarming slide=== | + | ====Swarming slide==== |
* Change the flagella of the bug | * Change the flagella of the bug | ||
* Zoom into the plates | * Zoom into the plates | ||
| - | ===Subtilin slide=== | + | ====Subtilin slide==== |
* Show subtilin only when the bug is at the end of the crack | * Show subtilin only when the bug is at the end of the crack | ||
* Fill up the crack with bugs | * Fill up the crack with bugs | ||
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* Change the pveg promoter to the oxygen sensitive promoter | * Change the pveg promoter to the oxygen sensitive promoter | ||
| - | ===Subtilin immunity slide=== | + | ====Subtilin immunity slide==== |
* SAY: Subtilin immunity ablready in the registry, that is why we use it | * SAY: Subtilin immunity ablready in the registry, that is why we use it | ||
* Mention what the individual CDS do | * Mention what the individual CDS do | ||
* Green tick for those that we did and a red cross for those that we did not | * Green tick for those that we did and a red cross for those that we did not | ||
| - | ===Workflow slide=== | + | ====Workflow slide==== |
* Change the word interesting to valuable to all synthetic biologist | * Change the word interesting to valuable to all synthetic biologist | ||
* Cut down on the script | * Cut down on the script | ||
| Line 47: | Line 45: | ||
* Integration of lots of data that is why we use e-science | * Integration of lots of data that is why we use e-science | ||
| - | ===YneA slide=== | + | ====YneA slide==== |
* Put filamentous cells into the pictures | * Put filamentous cells into the pictures | ||
* Metabolic pathway picture and link it | * Metabolic pathway picture and link it | ||
| - | ===Glue slide=== | + | ====Glue slide==== |
* The iGEM plates to go onto it | * The iGEM plates to go onto it | ||
| - | ===Kill switch=== | + | ====Kill switch==== |
* Can bin it | * Can bin it | ||
* Move to the ethic page | * Move to the ethic page | ||
| - | ===Ethic slide=== | + | ====Ethic slide==== |
* Move it to the front after the introduction and also mention in the summary | * Move it to the front after the introduction and also mention in the summary | ||
* What is the danger and how we make it safe | * What is the danger and how we make it safe | ||
| - | ===Others=== | + | ====Others==== |
* Show that the bug is able to grow in concrete | * Show that the bug is able to grow in concrete | ||
| - | ===Urease slide=== | + | ====Urease slide==== |
* Have to show that calcium carbonate is filling up the cracks | * Have to show that calcium carbonate is filling up the cracks | ||
* Have to say that we are better than the rest | * Have to say that we are better than the rest | ||
* Highlight the advantage of ours and the disadvantage of others | * Highlight the advantage of ours and the disadvantage of others | ||
* Mention SR1 | * Mention SR1 | ||
| - | ==Lab feedback== | + | |
| - | ===Filamentous cells=== | + | ===Lab feedback=== |
| + | ====Filamentous cells==== | ||
* Redo the microscopic | * Redo the microscopic | ||
* Correlate between the expression of GFP and the length | * Correlate between the expression of GFP and the length | ||
| - | ===SR1=== | + | ====SR1==== |
* We have colonies | * We have colonies | ||
| - | ===Scanning electron microscopy=== | + | ====Scanning electron microscopy==== |
* The concrete samples are already with the EM unit | * The concrete samples are already with the EM unit | ||
| - | ==Agenda== | + | ===Agenda=== |
| - | * To | + | * To re-edit the agenda |
| - | ==Action points== | + | ===Action points=== |
* '''Rachel''' and '''Phil''': Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry. | * '''Rachel''' and '''Phil''': Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry. | ||
* Everyone to do up the presentation slides first. | * Everyone to do up the presentation slides first. | ||
| - | ==Next meeting== | + | ===Next meeting=== |
| - | Wed | + | *13th, Wed October, 4 p.m. |
| - | + | *Chair: Steven, Computer: Alan, Minutes: Deena | |
| - | * | + | |
| - | + | ||
| - | + | ||
Revision as of 17:04, 25 October 2010
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Contents |
Formal Meeting - 6 October 2010
- Apologies: Colin Harwood, Jem and Phil
Presentation
Introduction
- Mention why do we use this bug and that it is able to live in high pH
- Have to menetion that we all biobrick have been modelled by SBML and copasi
Bacilla Filla slide
- The solution words to be smaller than the logo
- Logo have to be the biggest!
- Solution at the top of the page with a small logo below it then zoom into the logo
Technical review slide
- Show the link between the genes
- Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
- A single shot overview then to the biobricks
- SAY: This is a crack, and here is our bug on the surface of the crack, etc
- Label the pictures!
- Animation for the overview, ie, the background to remain the same
Swarming slide
- Change the flagella of the bug
- Zoom into the plates
Subtilin slide
- Show subtilin only when the bug is at the end of the crack
- Fill up the crack with bugs
- Describle the subtilin signalling system
- Change the pveg promoter to the oxygen sensitive promoter
Subtilin immunity slide
- SAY: Subtilin immunity ablready in the registry, that is why we use it
- Mention what the individual CDS do
- Green tick for those that we did and a red cross for those that we did not
Workflow slide
- Change the word interesting to valuable to all synthetic biologist
- Cut down on the script
- Have to make it fit to the presentation
- Integration of lots of data that is why we use e-science
YneA slide
- Put filamentous cells into the pictures
- Metabolic pathway picture and link it
Glue slide
- The iGEM plates to go onto it
Kill switch
- Can bin it
- Move to the ethic page
Ethic slide
- Move it to the front after the introduction and also mention in the summary
- What is the danger and how we make it safe
Others
- Show that the bug is able to grow in concrete
Urease slide
- Have to show that calcium carbonate is filling up the cracks
- Have to say that we are better than the rest
- Highlight the advantage of ours and the disadvantage of others
- Mention SR1
Lab feedback
Filamentous cells
- Redo the microscopic
- Correlate between the expression of GFP and the length
SR1
- We have colonies
Scanning electron microscopy
- The concrete samples are already with the EM unit
Agenda
- To re-edit the agenda
Action points
- Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
- Everyone to do up the presentation slides first.
Next meeting
- 13th, Wed October, 4 p.m.
- Chair: Steven, Computer: Alan, Minutes: Deena
