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Team:Newcastle/Meetings/21 October 2010
From 2010.igem.org
(Difference between revisions)
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*''B. subtilis'' transformation tommorrow | *''B. subtilis'' transformation tommorrow | ||
*Fluorescence | *Fluorescence | ||
| - | * | + | *Characterisation of previous part!!! |
==Concrete== | ==Concrete== | ||
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==Lab work== | ==Lab work== | ||
| - | *DNA submitted | + | *DNA submitted - tracking number sent |
*1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted! | *1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted! | ||
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*Phil - DON'T BE AGGRESSIVE!!!! | *Phil - DON'T BE AGGRESSIVE!!!! | ||
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*Beginning: | *Beginning: | ||
**say what the project is about | **say what the project is about | ||
Revision as of 16:26, 25 October 2010
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- Apologies - Phil, Younus, Deena late
- Action point: Phil and Rachel add characteristation data to the wiki
Contents |
yneA Characteristaion:
- ΔamyE:Pspac(hy)::yneA
- B. subtilis spread of IPTG may not be homogenous through the population.
- E. coli has transporter deltions: check E. coli wiki
- take out pMap65 results
- Median, Mode add to graph
- pMutin4 proportion of cells
- 20% responding- looks like
- Biomass of cells - length * amount (counts)) against IPTG
- Lysis acts to counter the overexpression of yneA ... so we have fewer cells
- Younus AP: send script for yneA to Jen, Matt, Neil
- Plot: Max cell length against ITPG + Error bars
Arabinose
- We think we have it cloned infront of GFP
- B. subtilis transformation tommorrow
- Fluorescence
- Characterisation of previous part!!!
Concrete
- Damaged building - earthquake
Flux balance
- difference between optimal and normal
Lab work
- DNA submitted - tracking number sent
- 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
- Deena and Harsh
- Risk assessment - Bacillus
- BioCOSH
- Safety Guy talk mention it
Presentation
- Phil - DON'T BE AGGRESSIVE!!!!
- Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- Previous work on CaCO3
- Don't use the word PROVES
- Separate slid for work flows
- Alan - Flux first, speak slowly
- Summary table- redo
- BRONZE, SILVER, GOLD!!!!!!!
- Gold
- Jannetta write RFC
- number on judging form
- webservice!!!
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- Images are ours
Other
- Insurance form
- Taxi
- Younus - Poster next thursday due in?
- Action point check presentation and poster submission!!!
   
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