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Team:Newcastle/Meetings/21 October 2010
From 2010.igem.org
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*Why bacillus? | *Why bacillus? | ||
*bioconcrete- show its current | *bioconcrete- show its current | ||
| - | + | *Non-pathogenic not non-virulent | |
| - | Non-pathogenic not non-virulent | + | *Previous work on CaCO<sub>3</sub> |
| - | Previous work on | + | *Don't use the word '''PROVES''' |
| - | Don't use the word '''PROVES''' | + | *Separate slid for work flows |
| - | Separate slid for work flows | + | *Alan - Flux first, speak slowly |
| - | + | *Summary table- redo | |
| - | Alan - Flux first, speak slowly | + | *BRONZE, SILVER, GOLD!!!!!!! (see below) |
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| - | Summary table- redo | + | |
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| - | BRONZE, SILVER, GOLD!!!!!!! | + | |
===GOLD=== | ===GOLD=== | ||
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Jannetta write RFC | Jannetta write RFC | ||
*number on judging form | *number on judging form | ||
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===SEM=== | ===SEM=== | ||
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Images are ours | Images are ours | ||
Revision as of 16:13, 25 October 2010
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Apologies - Phil, Younus, Deena late
Action point: Phil and Rachel add characteristation data to the wiki
Contents |
yneA Characteristaion:
- ΔamyE:Pspac(hy)::yneA
- Bacillus subtilis spread of IPTG may not be homogenous through the population.
- E.coli has transporter deltions: check E.coli wiki
take out pMap65 results
Median, Mode add to graph
pMutin4 proportion of cells
20% responding- looks like
Biomass of cells - length * amount (counts)) against IPTG
Lysis acts to counter the overexpression of yneA ... so we have fewer cells
Younus AP: send script for yneA to Jen, Matt, Neil
Plot: Max cell length against ITPG + Error bars
Arabinose
We think we have it cloned infront of GFP Bacillus subtilis transformation tommorrow Fluorescence Characterisation of previous part!!!
Concrete
Damaged building- earthquake
Flux balance
difference between optimal and normal
Lab work
DNA submitted... tracking number sent 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
Deena and Harsh
Risk assessment- Bacillus
BioCOSH
Safety Guy talk mention it
Presentation
Phil DON'T BE AGGRESSIVE!!!!
Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- Previous work on CaCO3
- Don't use the word PROVES
- Separate slid for work flows
- Alan - Flux first, speak slowly
- Summary table- redo
- BRONZE, SILVER, GOLD!!!!!!! (see below)
GOLD
Jannetta write RFC
- number on judging form
- webservice!!!
SEM
Images are ours
Other
Insurance form Taxi
Younus - Poster next thursday due in?
Action point check presentation and poster submission!!!
   
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