Team:Caltech/Week 3

Monday 6/28

• Tested the success of the construct we made using digest and gel electrophoresis.
  • Digested ligation product from 6/26 with EcoRI and PstI.
  • Ran digests on gel along with 1kb and 100kb molecular ladders.
• Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.

Image of a test gel of our third ligation product. Note that the middle two lanes are sadly empty.

• Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Tuesday 6/29

• Transformation results: Transformation of the ampicillin-resistant BioBricks seemed to be successful. However, no bacterial growth was present in the plates and liquid cultures with tetracyclin present. This seems to indicate a problem with the tetracyclin used.
  • Of the plates with bacterial growth ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100 (red)], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200 (orange)]), there was evidence of dye production, indicating a successful transformation.
• Transformed 7 more bricks (pigments & our AND gate components):

Wednesday 6/30

Thursday 7/1

Friday 7/2

Weekend 7/3-4