Team:Caltech/Week 3

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(New page: {{Caltech_iGEM_10| Content= Back to Notebook ==Monday 6/28== ==Tuesday 6/29== ==Wednesday 6/30== ==Thursday 7/1== ==Friday 7/2== ==Weekend 7/3-4==   }})
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==Monday 6/28==
==Monday 6/28==
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* Tested the success of the construct we made using digest and gel electrophoresis.
 +
** Digested ligation product from 6/26 with EcoRI and PstI.
 +
** Ran digests on gel along with 1kb and 100kb molecular ladders.
 +
* Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
 +
* Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
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** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]
==Tuesday 6/29==
==Tuesday 6/29==

Revision as of 00:21, 29 June 2010


iGEM 2010



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Back to Notebook

Contents

Monday 6/28

  • Tested the success of the construct we made using digest and gel electrophoresis.
    • Digested ligation product from 6/26 with EcoRI and PstI.
    • Ran digests on gel along with 1kb and 100kb molecular ladders.
  • Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
  • Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Tuesday 6/29

Wednesday 6/30

Thursday 7/1

Friday 7/2

Weekend 7/3-4

 
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