Team:Caltech/Week 1

From 2010.igem.org

(Difference between revisions)

Revision as of 23:52, 26 June 2010

iGEM 2010

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support

Back to Notebook

Monday 6/14

Discussed procuring items & lab space.
Prepared for SURF start.

Tuesday 6/15

Created GANTT Chart (right) detailing our work plan for the summer.

GANTT Chart diagramming our planned progress throughout the summer.

Detailed possible side projects, open to any member as time permits:
- Agarase
- pH/Environmental Response
- Phototaxis
- Colored plastic
- Activa (transglutaminase)
Procured supplies from stock room
Cleaned out lab space
Prepared LB-amp media & plates

Wednesday 6/16

Procured remaining antibiotics & sample of XL1 Blue cells
Prepare orders for Sigma, NEB, and others
- Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
- NEB: Biobrick assembly kit, Ladders, polymerase
Prepared list of biobricks to order
- Addl Lysis bricks
- Agarase bricks

Thursday 6/17

Made stock solutions for transformation and subsequent culturing
Transformed XL-1 Blue E. Coli with selected BioBricks
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
Ordered more BioBricks:
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112012 K112012]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K108001 K108001]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112806 K112806]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284001 K284001]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_I737020 I737020]

(We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.)

Friday 6/18

Checked transformation
Minipreped transformed bacteria
- Checked the plasmid concentration in the mini-prep solution
Made glycerol stocks from the transformed plasmid

Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found

@@ Line 33: / Line 33: @@
 **[http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
 * Ordered more BioBricks:
-** BBa_K112012
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112012 K112012]
-** BBa_K108001
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K108001 K108001]
-** BBa_K112806
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112806 K112806]
-** BBa_K284001
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284001 K284001]
-** BBa_I737020
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_I737020 I737020]
-(We also wanted to order sequencing primers G00100 & G00101, but apparently those aren't sent to teams.)
+(We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.)
 ==Friday 6/18==
 *Checked transformation