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Minnesota-experimental/23 June 2010
From 2010.igem.org
(Difference between revisions)
(New page: '''Annie''' Vector digestion with BglII/NotI. Placed in thirty seven degree incubator overnight. PCR product of Eut-C DsRed product ran on gel. Gel failed. '''Ian''' PacBB-GFP digest wi...) |
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'''Ian''' | '''Ian''' | ||
PacBB-GFP digest with BglII/NotI. Incubated overnight at thirty-seven degrees Celsius. | PacBB-GFP digest with BglII/NotI. Incubated overnight at thirty-seven degrees Celsius. | ||
| + | '''Tony''' | ||
| + | Backbone vector digestion with BglII/NotI. Set at thirty seven degrees Celsius for one hour. Second attempt of pucBB-EutG-GFP. Transformation of digestion overnight. | ||
| + | '''Matt''' | ||
| + | Digestion of pacBB-GFP with BglII/NotI. Incubated overnight at thirty seven degrees Celsius. Ran gel of Dsred-EutC reaction. Bands are blurry indicating failure. | ||
Latest revision as of 23:33, 23 October 2010
Annie Vector digestion with BglII/NotI. Placed in thirty seven degree incubator overnight. PCR product of Eut-C DsRed product ran on gel. Gel failed. Ian PacBB-GFP digest with BglII/NotI. Incubated overnight at thirty-seven degrees Celsius. Tony Backbone vector digestion with BglII/NotI. Set at thirty seven degrees Celsius for one hour. Second attempt of pucBB-EutG-GFP. Transformation of digestion overnight. Matt Digestion of pacBB-GFP with BglII/NotI. Incubated overnight at thirty seven degrees Celsius. Ran gel of Dsred-EutC reaction. Bands are blurry indicating failure.
