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From 2010.igem.org

Annie Vector digestion with BglII/NotI. Placed in thirty seven degree incubator overnight. PCR product of Eut-C DsRed product ran on gel. Gel failed. Ian PacBB-GFP digest with BglII/NotI. Incubated overnight at thirty-seven degrees Celsius. Tony Backbone vector digestion with BglII/NotI. Set at thirty seven degrees Celsius for one hour. Second attempt of pucBB-EutG-GFP. Transformation of digestion overnight. Matt Digestion of pacBB-GFP with BglII/NotI. Incubated overnight at thirty seven degrees Celsius. Ran gel of Dsred-EutC reaction. Bands are blurry indicating failure.