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Minnesota-experimental/2 March 2010
From 2010.igem.org
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(New page: '''Rachel''' Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microlit...)
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(New page: '''Rachel''' Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microlit...)
Newer edit →
Revision as of 19:34, 23 October 2010
Rachel Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microliter reaction. Tony and Matt Concentrated Eut LK solution in speed-vac for sixteen minutes. Eut LK solution digested with BglII/NotI (50 microliter reaction). Rachel and Ian Transformation of 15 microliters of ligation reaction from 3/2. Plated on amp plates at 37 degree overnight.
