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UTDallas/19 October 2010
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(New page: *Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length *Digested the random part (BBa_J04450) with XbaI and...) |
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| + | ==Notebook== | ||
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| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=06}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=07}} | ||
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| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=09}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=10}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=11}} | ||
| + | |} | ||
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| + | ===October 19, 2010 === | ||
*Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length | *Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length | ||
*Digested the random part (BBa_J04450) with XbaI and PstI to get just the backbone, pSB1C3, then ran gel electrophoresis (took only the top band ~2050bp) and ran gel purification: results were good. | *Digested the random part (BBa_J04450) with XbaI and PstI to get just the backbone, pSB1C3, then ran gel electrophoresis (took only the top band ~2050bp) and ran gel purification: results were good. | ||
Revision as of 23:23, 21 October 2010
Notebook
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October 19, 2010
- Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length
- Digested the random part (BBa_J04450) with XbaI and PstI to get just the backbone, pSB1C3, then ran gel electrophoresis (took only the top band ~2050bp) and ran gel purification: results were good.
- Both parts were ran on the same gel. The test digestion is the left three bands and the pSB1C3 is the right bands.
- Ligated the pSB1C3 to PyeaR+GFP and PyeaR+RFP
- Incubated GFP overnight for miniprepping and digestion tomorrow to ligate to the alkane sensor
