UTDallas/19 October 2010

From 2010.igem.org


Notebook

June
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


October 19, 2010

Nitrate Sensor
  • Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length
  • Digested the random part (BBa_J04450) with XbaI and PstI to get just the backbone, pSB1C3, then ran gel electrophoresis (took only the top band ~2050bp) and ran gel purification: results were good.
  • Both parts were ran on the same gel. The test digestion is the left three bands and the pSB1C3 is the right bands.
  • Ligated the pSB1C3 to PyeaR+GFP and PyeaR+RFP
  • Incubated GFP overnight for miniprepping and digestion tomorrow to ligate to the alkane sensor
Alkane Sensor
  • Midi prep on the overnight grown cells having part E0240.