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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
From 2010.igem.org
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=2010/10/15= | =2010/10/15= | ||
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===1：DNA extraction=== | ===1：DNA extraction=== | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
*vector | *vector | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
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<Sample><br /> | <Sample><br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
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<Result><br /> | <Result><br /> | ||
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＜Member＞<br /> | ＜Member＞<br /> | ||
Mariko | Mariko | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | + | ||
(After electrophoresis) | (After electrophoresis) | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
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<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
*vector | *vector | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
<Protocol><br /> | <Protocol><br /> | ||
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<Sample><br /> | <Sample><br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
*vector | *vector | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
| Line 85: | Line 77: | ||
<Sample><br /> | <Sample><br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
<Protocol><br /> | <Protocol><br /> | ||
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＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
*vector | *vector | ||
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
| Line 109: | Line 100: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *6-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
| Line 124: | Line 114: | ||
<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9 |
*9-7 | *9-7 | ||
*1-2-3 | *1-2-3 | ||
Revision as of 12:40, 19 October 2010
Contents |
2010/10/15
1：DNA extraction
＜Member＞
Hitomi
＜Sample＞
＜Protocol＞
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
<Protocol>
See Protocol 8
<Result>
3：DNA extraction from gels
＜Member＞
Mariko
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
4:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
6:PCR
<Member>
mio
<Sample>
<Protocol>
See Protocol 2
7：DNA Digestion
＜Member＞
Hitomi
＜Sample, Materials＞
- vector
- 9-7
＜Protocol＞
See Protocol 9
8：DNA Ligation
＜Member＞
Hitomi
＜Sample＞
＜Protocol＞
See Protocol 3
9:PCR
<Member>
mio
<Sample>(temperature in annealing:℃)
- [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9
- 9-7
- 1-2-3
- 4-5
- 6-7
- 9-7
<Protocol>
See Protocol 2
10:PCR
<Member>
nito,mizuki
<Sample>
- 9
- vector
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio
<Sample>
- 9
<Protocol>
See Protocol 8
<Result>
12:Transformation
<Member>
nito
<Sample>
- 7-8-10
- 1-2-7
- 10-2-7
- JM109
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
nito
<Sample>
- 1-2-3
- 4-5
- 6-7
- 9-7
- 1
<Protocol>
See Protocol 8
<Result>
14:Electrophoresis
<Member>
nito
<Sample>
- 9
- vector
<Protocol>
See Protocol 8
<Result>
