Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/08

From 2010.igem.org

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(2:Electrophoresis)
(1:PCR①)
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<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
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*9
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
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*12
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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*210
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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===2:Electrophoresis===
===2:Electrophoresis===

Revision as of 12:04, 19 October 2010


Contents

2010/10/

1:PCR①

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 1

2:Electrophoresis

<Member>
nito

<Sample>
PCR products

  • 9
  • 12
  • 210

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-08 16hr 46min.JPG

3:DNA Ligation

<Member>
Hitomi

<Sample>

  • 12
  • 45
  • 210

(after digestion)

<Protocol>
See Protocol 10


4:PCR②

<Member>
nito

<Sample>(temperature in annealing:℃)

  • 7
  • 9

<Protocol>
See Protocol 1


5:DNA extraction from gels

<Member>
nito

<Sample>
Digestion products

  • 12
  • 210

(After electrophoresis)

<Protocol>
See Protocol 4