Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/04
From 2010.igem.org
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<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
- | Ligated 10/3,10/4 | + | Ligated products 10/3,10/4 |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
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===4:PCR=== | ===4:PCR=== |
Revision as of 11:56, 19 October 2010
Contents |
2010/10/04
1:DNA extraction
<Member>
nito
<Sample>
Digestion products (10/3)
<Protocol>
See Protocol 4
2:DNA Ligation
<Member>
nito,Hitomi
<Sample>
(after digestion)
<Protocol>
See Protocol 10
3:PCR
<Member>
nito,Takahiro
<Sample>(temperature in annealing:℃)
Ligated products 10/3,10/4
<Protocol>
See Protocol 1
4:PCR
<Member>
Mariko,Takahiro,nito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1