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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/29
From 2010.igem.org
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
PCR products | PCR products | ||
| - | *1 (9/27)① | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (9/27)① |
| - | *1 (9/27)② | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (9/27)② |
| - | *2 (9/27) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/27) |
| - | *3 (9/27) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] (9/27) |
| - | *4 (9/27)① | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/27)① |
| - | *4 (9/27)② | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/27)② |
| - | *5 (9/27) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5](9/27) |
| - | *8 (9/27) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (9/27) |
| - | *10(9/27) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10](9/27) |
Digestion products (9/27) | Digestion products (9/27) | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
| - | |||
===2:Electrophoresis=== | ===2:Electrophoresis=== | ||
Revision as of 11:46, 19 October 2010
Contents |
2010/09/29
1：DNA extraction
＜Member＞
nito
＜Sample＞
PCR products
Digestion products (9/27)
＜Protocol＞
See Protocol 4
2:Electrophoresis
<Member>
nito
<Sample>
PCR products (9/27)
- 1
- 2
- 3
- 4
<Protocol>
See Protocol 8
<Result>
3：DNA Ligation
＜Member＞
nito
＜Sample＞
- 1,2
- 4,5
(after digestion)
＜Protocol＞
See Protocol 3
4:PCR
<Member>
mio,mizuki
<Sample>(temperature in annealing:℃)
- 5 (70)
- 6 (63)
- 7-8(70)
- 9 (58,63,69,70,72)
- 10 (58)
- 1-2(72)
<Protocol>
See Protocol 1
But today we used Kod.
