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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17
From 2010.igem.org
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(→5：DNA extraction from gels) |
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *7(9/17) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7](9/17) |
| - | *8(9/17) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8](9/17) |
(after digestion) | (after digestion) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
Revision as of 11:30, 19 October 2010
Contents |
2010/09/17
1:PCR
<Member>
mio
<Sample>
(temperature in annealing)
<Protocol>
See Protocol 1
2：DNA Digestion
＜Member＞
mizuki
＜Sample, Materials＞
・PCR productions
・Digest enzyme
- 1,4,8 : AvrⅡ
- 2,7 : NheⅠ
- 5L : SpeⅠ
＜Protocol＞
See Prptpcpl 9
3:Electrophoresis①
<Member>
mio
<Sample>
PCR products (9/17)
<Protocol>
See Protocol 8
<Result>
4:Electrophoresis②
<Member>
mizuki
<Sample>
Digestion products (9/17)
<Protocol>
See Protocol 8
<Result>
5：DNA extraction from gels
＜Member＞
mizuki
＜Sample＞
Digestion products(9/17)
(After electrophoresis)
＜Protocol＞
See Protocol 4
6：DNA Ligation
＜Member＞
mizuki
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
