Team:Newcastle/Meetings/6 October 2010

From 2010.igem.org

(Difference between revisions)
(Roll call)
(6 October 2010 Meeting)
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{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
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=6 October 2010 Meeting=
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==Formal Meeting - 6 October 2010==
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==Roll call==
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* Apologies: Colin Harwood, Jem and Phil
* Apologies: Colin Harwood, Jem and Phil
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==Presentation==
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===Presentation===
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===Introduction===
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====Introduction====
* Mention why do we use this bug and that it is able to live in high pH
* Mention why do we use this bug and that it is able to live in high pH
* Have to menetion that we all biobrick have been modelled by SBML and copasi
* Have to menetion that we all biobrick have been modelled by SBML and copasi
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===Bacilla Filla slide===
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====Bacilla Filla slide====
* The solution words to be smaller than the logo
* The solution words to be smaller than the logo
* Logo have to be the biggest!
* Logo have to be the biggest!
* Solution at the top of the page with a small logo below it then zoom into the logo
* Solution at the top of the page with a small logo below it then zoom into the logo
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===Technical review slide===
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====Technical review slide====
* Show the link between the genes
* Show the link between the genes
* Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
* Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
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* Animation for the overview, ie, the background to remain the same
* Animation for the overview, ie, the background to remain the same
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===Swarming slide===
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====Swarming slide====
* Change the flagella of the bug
* Change the flagella of the bug
* Zoom into the plates
* Zoom into the plates
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===Subtilin slide===
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====Subtilin slide====
* Show subtilin only when the bug is at the end of the crack
* Show subtilin only when the bug is at the end of the crack
* Fill up the crack with bugs
* Fill up the crack with bugs
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* Change the pveg promoter to the oxygen sensitive promoter
* Change the pveg promoter to the oxygen sensitive promoter
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===Subtilin immunity slide===
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====Subtilin immunity slide====
* SAY: Subtilin immunity ablready in the registry, that is why we use it
* SAY: Subtilin immunity ablready in the registry, that is why we use it
* Mention what the individual CDS do
* Mention what the individual CDS do
* Green tick for those that we did and a red cross for those that we did not
* Green tick for those that we did and a red cross for those that we did not
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===Workflow slide===
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====Workflow slide====
* Change the word interesting to valuable to all synthetic biologist
* Change the word interesting to valuable to all synthetic biologist
* Cut down on the script
* Cut down on the script
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* Integration of lots of data that is why we use e-science
* Integration of lots of data that is why we use e-science
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===YneA slide===
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====YneA slide====
* Put filamentous cells into the pictures
* Put filamentous cells into the pictures
* Metabolic pathway picture and link it
* Metabolic pathway picture and link it
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===Glue slide===
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====Glue slide====
* The iGEM plates to go onto it
* The iGEM plates to go onto it
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===Kill switch===
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====Kill switch====
* Can bin it  
* Can bin it  
* Move to the ethic page
* Move to the ethic page
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===Ethic slide===
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====Ethic slide====
* Move it to the front after the introduction and also mention in the summary
* Move it to the front after the introduction and also mention in the summary
* What is the danger and how we make it safe
* What is the danger and how we make it safe
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===Others===
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====Others====
* Show that the bug is able to grow in concrete
* Show that the bug is able to grow in concrete
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===Urease slide===
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====Urease slide====
* Have to show that calcium carbonate is filling up the cracks
* Have to show that calcium carbonate is filling up the cracks
* Have to say that we are better than the rest
* Have to say that we are better than the rest
* Highlight the advantage of ours and the disadvantage of others
* Highlight the advantage of ours and the disadvantage of others
* Mention SR1
* Mention SR1
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==Lab feedback==
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===Filamentous cells===
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===Lab feedback===
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====Filamentous cells====
* Redo the microscopic  
* Redo the microscopic  
* Correlate between the expression of GFP and the length
* Correlate between the expression of GFP and the length
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===SR1===
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====SR1====
* We have colonies
* We have colonies
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===Scanning electron microscopy===
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====Scanning electron microscopy====
* The concrete samples are already with the EM unit
* The concrete samples are already with the EM unit
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==Agenda==
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===Agenda===
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* To reedit the agenda
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* To re-edit the agenda
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==Action points==
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===Action points===
* '''Rachel''' and '''Phil''': Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
* '''Rachel''' and '''Phil''': Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
* Everyone to do up the presentation slides first.
* Everyone to do up the presentation slides first.
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==Next meeting==
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===Next meeting===
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Wed 4pm.
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*13th, Wed October, 4 p.m.
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*Chair: Steven, Computer: Alan, Minutes: Deena
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*'''Chair:''' Steven
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*'''Computer:''' Alan
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*'''Minutes:''' Deena
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Revision as of 17:04, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Formal Meeting - 6 October 2010

  • Apologies: Colin Harwood, Jem and Phil

Presentation

Introduction

  • Mention why do we use this bug and that it is able to live in high pH
  • Have to menetion that we all biobrick have been modelled by SBML and copasi

Bacilla Filla slide

  • The solution words to be smaller than the logo
  • Logo have to be the biggest!
  • Solution at the top of the page with a small logo below it then zoom into the logo

Technical review slide

  • Show the link between the genes
  • Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
  • A single shot overview then to the biobricks
  • SAY: This is a crack, and here is our bug on the surface of the crack, etc
  • Label the pictures!
  • Animation for the overview, ie, the background to remain the same

Swarming slide

  • Change the flagella of the bug
  • Zoom into the plates

Subtilin slide

  • Show subtilin only when the bug is at the end of the crack
  • Fill up the crack with bugs
  • Describle the subtilin signalling system
  • Change the pveg promoter to the oxygen sensitive promoter

Subtilin immunity slide

  • SAY: Subtilin immunity ablready in the registry, that is why we use it
  • Mention what the individual CDS do
  • Green tick for those that we did and a red cross for those that we did not

Workflow slide

  • Change the word interesting to valuable to all synthetic biologist
  • Cut down on the script
  • Have to make it fit to the presentation
  • Integration of lots of data that is why we use e-science

YneA slide

  • Put filamentous cells into the pictures
  • Metabolic pathway picture and link it

Glue slide

  • The iGEM plates to go onto it

Kill switch

  • Can bin it
  • Move to the ethic page

Ethic slide

  • Move it to the front after the introduction and also mention in the summary
  • What is the danger and how we make it safe

Others

  • Show that the bug is able to grow in concrete

Urease slide

  • Have to show that calcium carbonate is filling up the cracks
  • Have to say that we are better than the rest
  • Highlight the advantage of ours and the disadvantage of others
  • Mention SR1

Lab feedback

Filamentous cells

  • Redo the microscopic
  • Correlate between the expression of GFP and the length

SR1

  • We have colonies

Scanning electron microscopy

  • The concrete samples are already with the EM unit

Agenda

  • To re-edit the agenda

Action points

  • Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
  • Everyone to do up the presentation slides first.

Next meeting

  • 13th, Wed October, 4 p.m.
  • Chair: Steven, Computer: Alan, Minutes: Deena