Team:Tsinghua/project/outline
From 2010.igem.org
(New page: =BACKGROUND= Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same, made by identical immune cells that are all clones of a unique parent cell. When stimulated...) |
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Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same, made by identical immune cells that are all clones of a unique parent cell. When stimulated by almost any type of antigen, the immune system can create the specific antibody. This lays the foundation for monoclonal artificial antibodies. | Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same, made by identical immune cells that are all clones of a unique parent cell. When stimulated by almost any type of antigen, the immune system can create the specific antibody. This lays the foundation for monoclonal artificial antibodies. | ||
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Revision as of 14:01, 12 October 2010
Contents |
Project Outline
Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria. Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody. In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:
MODULE I: Generation of Antibody Library
MODULE II: Selection of Specific Antibody
Module I
Abstract
Flow chart
Landing Pad Insertion
Helper Plasmid(HP) Insertion
Donor Plasmid(DP) Construction
Strategy 1
Strategy 2
Donor Plasmid(DP) Insertion & Recombination Induction
Removal of Helper Plasmid(HP)
Module II
Strategy 1
Strategy 2
Strategy 3
Cooperation with Macquarie Australia