Team:Peking

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<a href="https://2010.igem.org/Team:Peking/Project/Biosensor"><font size=5><font color=#585858>Biosensor:</font></font></a><br>
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Based on reverse engineering principles, we engineered our bacteria into valid bioreporters for Hg(II) in aquatic environment. Rational design of genetic circuit topology was conducted to confer mercury sensor and regulator components high efficiency and robustness. Additionally, a new approach based on the use of multiple bioreporter cell lines to control assay variations and to improve in field application ease was also developed.
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<a href="https://2010.igem.org/Team:Peking/Project/Bioabsorbent"><font size=5><font color=#585858>Bioabsorbent:</font></font></a><br>
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A closer look into mercury- and lead- responsive regulators was conducted via 3D structure modeling, followed by rational design of metal binding peptides (MBP) for various heavy metals. Mercury and lead MBPs were accomplished in our bioware experiment. Then high-performance whole-cell bioabsorbent was constructed by expression of MBP on outer membrane surface, periplasm and cytosol of E.coli cells, verified by following function test.
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<a href="https://2010.igem.org/Team:Peking/Project/Expansion"><font size=3><font color=#585858>Expansion</font></font></a>:&nbsp;&nbsp;
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MerR family TFs share a high homology at <br> their metal binding domains, which implies that our strategies of bioreporter and bioabsorbent engineering might be applicable to other cases. Then our reverse engineering strategy was expanded to another common toxic heavy metal, lead (Pb) to prove the validness.
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<a href="https://2010.igem.org/Team:Peking/Project/Application"><font size=3><font color=#585858>Application</font></font></a>:&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;Traditional bioreporters are not practical when it <br>comes to in field application. One crucial factor is the preservation condition of the bacteria, often requiring costly instrument. We endeavo-<br>-red to solve this hard truth by development of a standard preservation method which endows our bioreporters with application ease.
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<a class="B1" a href="https://2010.igem.org/Team:Peking/Project/Biosensor">Biosensor<img src="https://static.igem.org/mediawiki/2010/1/1b/Sensorintro.png"/></a>
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<a class="B4" a href="https://2010.igem.org/Team:Peking/Project/Expansion">Expansion<img src="https://static.igem.org/mediawiki/2010/b/b1/Expansionintro.png"/></a>
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Revision as of 04:52, 17 October 2010

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Parts

    Our team submitted a library of thoroughly characterized and standardized parts. Therefore contributing an alternative set of tools towards heavy metal detection and decontamination in the iGEM context. What's more, as our MerR-family-derivated engineering method could be applicable in nearly all cases of heavy metal, our parts and corresponding documents provide a streamlined method for heavy metal bioreporter and bioabsorbent construction.

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Team

    Eighteen students and three instructors are working on our project during this summer. We split up into several subgroups whose focus and results you can follow on the Notebook or Project pages. If you want to know more about the subgroups and the people involved, meet us on our Team page.


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Modeling

Modeling     We adopt the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analysis whether they fit the objection function or not, thus getting the right topology and the proper range of parameters for the designation of our project.
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Human Practice

Biosafety     DNA recombination, Fragment synthesis, Bio-security... these are words that could hardly be ignored when doing researches of synthetic biology, which promises a great many improvements to our understanding of this world. The potential bio-security risk, however, is just around corner.
    Gene horizontal transfer, for example, is worth special attention. This summer, we did a thorough research on this topic, aiming at proactively finding out the current situation in China, a beginner in synthetic biology, and raised the awareness of researchers on such issues.
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