Team:Lethbridge/Notebook/Lab Work/October

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Revision as of 01:15, 8 October 2010

Feel free to look around our notebook!

Here you can check out the work we have done in the lab, click on a month to take a look!

Contents

1 October

1.1 October 2, 2010

1.1.1 ADS

1.2 October 5, 2010

1.2.1 ADS

October

October 2, 2010

ADS

Objective: Purify plasmid DNA from transformed Mr. GENE synthesized DNA

<partinfo>K331022</partinfo>
<partinfo>K331023</partinfo>
<partinfo>K331024</partinfo>
<partinfo>K331025</partinfo>

Method: Use Qiagen miniprep method with BioBasic EZ-10 Spin columns.

Objective: Create 20 new biobricks using recently arrived synthesized DNA and terminator <partinfo>B0015</partinfo>, pLac <partinfo>R0010</partinfo>, and pTet <partinfo>R0040</partinfo> to create the following new biobricks:

1)R0010 + K331022 to obtain <partinfo>K331026</partinfo>
2)R0040 + K331022 to obtain <partinfo>K331030</partinfo>
3)K331022 + B0015 to obtain <partinfo>K331034</partinfo>
4)K331022 + K331024 to obtain (NAME BIOBRICK!!!!)
5)R0010 + K331023 to obtain <partinfo>K331027</partinfo>
6)R0040 + K331023 to obtain <partinfo>K331031</partinfo>
7)K331023 + B0015 to obtain <partinfo>K331035</partinfo>
8)K331023 + K331025 to obtain (NAME BIOBRICK!!!!)
9)R0010 + K331024 to obtain <partinfo>K331028</partinfo>
10)R0040 + K331024 to obtain <partinfo>K331032</partinfo>
11)K331024 + B0015 to obtain <partinfo>K331036</partinfo>
12)K331024 + K331022 to obtain (NAME BIOBRICK!!!!)
13)R0010 + K331025 to obtain <partinfo>K331029</partinfo>
14)R0040 + K331025 to obtain <partinfo>K331033</partinfo>
15)K331025 + B0015 to obtain <partinfo>K331037</partinfo>
16)K331025 + K331023 to obtain (NAME BIOBRICK!!!!)
17)K331022 to obtain <partinfo>K331022</partinfo>
18)K331023 to obtain <partinfo>K331023</partinfo>
19)K331024 to obtain <partinfo>K331024</partinfo>
20)K331025 to obtain <partinfo>K331025</partinfo>

All parts will be inserted into pSB1C3.
Method: Use BioBrick Assembly Method
Results: Obtained RESULTS!!! positive colonies.

October 5, 2010

ADS

Objective: Purify plasmid DNA from successfully assembled biobricks.
Method: Use Qiagen miniprep method with BioBasic EZ-10 columns.
Will send these minipreps for sequencing at Macrogen.

@@ Line 213: / Line 213: @@
 ------------------------------------------------------------------------------------------------------------------------
 <b>Objective:</b> Create 20 new biobricks using recently arrived synthesized DNA and terminator <partinfo>B0015</partinfo>, pLac <partinfo>R0010</partinfo>, and pTet <partinfo>R0040</partinfo> to create the following new biobricks:
-*1)R0010 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*1)R0010 + K331022 to obtain <partinfo>K331026</partinfo>
-*2)R0040 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*2)R0040 + K331022 to obtain <partinfo>K331030</partinfo>
-*3)K331022 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*3)K331022 + B0015 to obtain <partinfo>K331034</partinfo>
 *4)K331022 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
-*5)R0010 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*5)R0010 + K331023 to obtain <partinfo>K331027</partinfo>
-*6)R0040 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*6)R0040 + K331023 to obtain <partinfo>K331031</partinfo>
-*7)K331023 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*7)K331023 + B0015 to obtain <partinfo>K331035</partinfo>
 *8)K331023 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
-*9)R0010 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*9)R0010 + K331024 to obtain <partinfo>K331028</partinfo>
-*10)R0040 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*10)R0040 + K331024 to obtain <partinfo>K331032</partinfo>
-*11)K331024 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*11)K331024 + B0015 to obtain <partinfo>K331036</partinfo>
 *12)K331024 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
-*13)R0010 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*13)R0010 + K331025 to obtain <partinfo>K331029</partinfo>
-*14)R0040 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*14)R0040 + K331025 to obtain <partinfo>K331033</partinfo>
-*15)K331025 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*15)K331025 + B0015 to obtain <partinfo>K331037</partinfo>
 *16)K331025 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
-*17)K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*17)K331022 to obtain <partinfo>K331022</partinfo>
-*18)K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*18)K331023 to obtain <partinfo>K331023</partinfo>
-*19)K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*19)K331024 to obtain <partinfo>K331024</partinfo>
-*20)K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
+*20)K331025 to obtain <partinfo>K331025</partinfo>
 All parts will be inserted into pSB1C3.<br>
 <b>Method:</b> Use BioBrick Assembly Method <br>